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KMID : 0370220230670060398
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Yakhak Hoeji
2023 Volume.67 No. 6 p.398 ~ p.403
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Study for Anti-inflammatory Effects of Centella asiatica Extract Powder
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Bae Jae-Pil
Lee Ki-Man
Venkata Prakash Annamneedi
Park Jun-Woo
Park Jin-Hong
Kang Tae-Jin
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Abstract
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Centella asiatica (C. asiatica) is currently widely used in wound healing and has recently been reported to haveimmune suppressive properties. This study investigated the anti-inflammatory effect with ethanol-extract of C. asiaticacultivated in a smart farm. Analysis of asiaticoside in C. asiatica extract was performed using high performance liquidchromatography (HPLC) and as a result, the asiaticoside content of C. asiatica extract was 23.17 mg/g. The effect of C.
asiatica extract on the expression of inflammatory mediators, such as interleukin-1beta (IL-1β), IL-6 and tumor necrosisfactor-alpha (TNF-α), and nitric oxide (NO) was examined in murine macrophage cell line, RAW264.7 using ELISAmethod. C. asiatica extract inhibited the production of NO, IL-1β, IL-6, and TNF-α in macrophages stimulated withlipopolysaccharide (LPS) at a dose dependent manner. C. asiatica extract also suppressed the expression of IL-6 and IL-8 in HaCaT cells, a human keratinocytes, stimulated with TNF-α/IFN-?. These results suggest that C. asiatica cultivatedin a smart farm can be effectively utilized as a therapeutic agent for inflammatory disease.
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KEYWORD
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Centella asiatica extract, Smart farm, Inflammation, Cytokine, Health functional food, in vitro
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